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Fig. 3 | Skeletal Muscle

Fig. 3

From: Expression patterns of FSHD-causing DUX4 and myogenic transcription factors PAX3 and PAX7 are spatially distinct in differentiating human stem cell cultures

Fig. 3

RNA expression profiles of myogenic regulators PAX3 and PAX7 and DUX4 expression in cultures of human ES cells and iPS cell clones during myogenic differentiation. RNA was isolated at five time points (D0, D7, D21, D30, and D40) representing early, middle, and late stages of myogenesis. Quantitative PCR of PAX3, PAX7, and DUX4 RNA transcripts was performed at each time point, and values normalized to GAPDH RNA levels in the same preparations. Oligonucleotide primers designed to amplify the 3′ end of the DUX4 gene from the terminal D4Z4 unit on chromosome 4 were used to quantify DUX4 transcripts. Undetected RNA transcripts are marked with an arrow

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Skeletal Muscle

ISSN: 2044-5040

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