Table 2 Frequency of PAX3/DUX4 and PAX7/DUX4 double positive nuclei in myogenic cultures
Cells | TF | aNumber of positive cells at indicated time | Observed double + frequency (D40) | Expected double + frequency (D40) | |||||
|---|---|---|---|---|---|---|---|---|---|
D7 | D21 | D30 | D40 | PAX3/DUX4 | PAX7/DUX4 | PAX7/DUX4 | # p value | ||
hESC-Cntrl | PAX7 | 0 | 56 | 20 | 54 | <1/150 | <1/54 | 0 | na |
PAX3 | 22 | 68 | 60 | 0 | |||||
DUX4 | 0 | 0 | 0 | 0 | |||||
Dapi | nd | nd | nd | 491 | |||||
hESC-FSHD | PAX7 | 0 | 325 | 289 | 373 | <1/82 | <1/373 | 1/605 | .098 |
PAX3 | 52 | 20 | 10 | 0 | |||||
DUX4 | 0 | 0 | 0 | 6 | |||||
Dapi | nd | nd | nd | 1164 | |||||
hiPSC-mosaic1-long | PAX7 | 0 | 360 | 320 | 1456 | <1/565 | <1/1456 | 0 | na |
PAX3 | 75 | 458 | 32 | 0 | |||||
DUX4 | 0 | 0 | 0 | 0 | |||||
Dapi | nd | nd | nd | 2873 | |||||
hiPSC-mosaic1-short | PAX7 | 0 | 40 | 115 | 70 | <1/566 | <1/70 | 1/114,753 | .958 |
PAX3 | 84 | 359 | 123 | 0 | |||||
DUX4 | 0 | 0 | 0 | 3 | |||||
Dapi | nd | nd | nd | 4909 | |||||
hiPSC-mosaic2-long | PAX7 | 0 | 8 | 45 | 127 | <1/162 | <1/127 | 0 | na |
PAX3 | 110 | 35 | 17 | 0 | |||||
DUX4 | 0 | 0 | 0 | 0 | |||||
Dapi | nd | nd | nd | 410 | |||||
hiPSC-mosaic2-short | PAX7 | 0 | 19 | 153 | 1678 | <1/102 | <1/1678 | 1/276 | <.0001 |
PAX3 | 93 | 5 | 4 | 0 | |||||
DUX4 | 0 | 0 | 0 | 43 | |||||
Dapi | nd | nd | nd | 4460 | |||||