Fig. 7

Myostatin signaling in the gastrocnemius and soleus of bGH (n = 9) and WT (n = 9) mice. Mature and precursor myostatin protein content in the gastrocnemius (a) and soleus (b) of WT and bGH mice. Phosphorylation of Smad2 (Ser245/250/255) and (Ser465/467) in the gastrocnemius (c) and soleus (d) of WT and bGH mice. Phosphorylation of Smad3 (Ser 423/425) and AcvR2B total protein in the gastrocnemius (e) and soleus (f) of WT and bGH mice. Relationship between Precursor Myostatin and STAT5 phosphorylation in gastrocnemius of bGH and WT skeletal muscle (g). Relationship between relative gastrocnemius mass and precursor myostatin in the gastrocnemius of bGH and WT skeletal muscle (h). Representative blots in the gastrocnemius and soleus of WT and bGH mice (i). Values in A.U. were normalized to a control sample run on each blot and then presented relative to GAPDH. Data are expressed as mean ± SEM. * P < 0.05, **P < 0.01, ***P < 0.005 ****P < 0.0005 vs. WT control mice. Gast, gastrocnemius; Sol, soleus