Fig. 5

Additional skeletal muscle histological features. Example of vascular leak in area of damage within mdx-ApoE Masson’s trichrome stained triceps brachii (yellow arrow indicates blood vessel and green arrows indicate red blood cells in muscle tissue), scale bars 100 μm (a). Ectopic bone (left) and calcification (right) in 4-month-old mdx-ApoE gastrocnemius stained with Alizarin red, scale bars 200 μm (b). Lipid deposits in mdx (c) and mdx-ApoE (d) oil red O stained triceps brachii, scale bars 500 μm (left) and 100 μm (right). Quantification of inflammation area (e) and calcification area (f) at 7 months of age on Western diet. Quantification of Evan’s blue dye extravasation in gastrocnemius (g) and quadriceps femoris (h) muscles of chow-fed wild-type, mdx and mdx-ApoE mice. Chow: WT (n = 5), mdx (n = 8), and mdx-ApoE (n = 9). Western: WT (n = 9), ApoE (n = 8), mdx (n = 5), and mdx-ApoE (n = 9). Mean + SEM *P < 0.05 **P < 0.01 ***P < 0.001 ****P < 0.0001