Fig. 1

Skeletal muscle of Fmr1 ^−/− mice have reduced numbers of satellite cells. a Immunostaining of PAX7 (green) on newly isolated single EDL myofibers isolated from adult wild-type (upper panel) and Fmr1 ^−/− (lower panel) mice. b Numbers of PAX7-positive satellite cells per EDL myofiber isolated from wild-type (white) and Fmr1 ^−/− (gray) mice. c Immunostaining of PAX7 (red) and bungarotoxin (Btx) labeling of neuromuscular junctions (NMJ) on single EDL myofibers isolated from wild-type and Fmr1 ^−/− mice. d Numbers of NMJs per EDL myofiber. e Levels of Pax7, Myf5, and MyoD transcripts, relative to Actb, in satellite cells isolated from adult muscle of wild-type (wt, black) and Fmr1 ^−/− (red) mice. f Fraction of PAX7(+) satellite cells that immunostain for MYF5 on single EDL myofibers isolated from muscle of wild-type (wt) and Fmr1 ^−/− mice. g Fraction of PAX7(+) satellite cells that immunostain for MYOD on single EDL myofibers isolated from muscle of wild-type (wt) and Fmr1 ^−/− mice. All values indicate mean (n ≥ 3) ± s.e.m. *p < 0.05, ***p < 0.001. ns not significant