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Fig. 4 | Skeletal Muscle

Fig. 4

From: Fragile X mental retardation protein regulates skeletal muscle stem cell activity by regulating the stability of Myf5 mRNA

Fig. 4

Satellite cells isolated from Fmr1 −/− mice have reduced the capacity to self-renew and contribute to the satellite cell pool after intramuscular engraftment into a mouse model of Duchenne muscular dystrophy. a Schematic representation of cell engraftment. b Immunostaining dystrophin (green) and PAX7 (red) on transverse sections 21 days after engraftment of satellite cells that had been newly isolated from muscle of Pax3 GFP/+ (wild type; left) and Pax3 GFP/+ ; Fmr1 −/− (Fmr1 −/−; right) mice. Arrowheads indicate location of satellite cells. Dotted lines indicate position of dystrophin-negative myofibers. Scale bars (white) indicate 50 μm. c Numbers of dystrophin-positive myofibers 21 days after engraftment of 10,000 satellite cells isolated from Pax3 GFP/+ (wt) and Pax3 GFP/+ ; Fmr1 −/− (Fmr1 −/−) mice. d Immunostaining GFP (green) and PAX7 (red) on transverse sections 21 days after engraftment of satellite cells from muscle of Pax3 GFP/+ (wild-type; left) and Pax3 GFP/+ ; Fmr1 −/− (Fmr1 −/−; right) mice. Arrowheads indicate location of GFP(+) satellite cells of donor origin. e Numbers of PAX7-positive and GFP-positive satellite cells of donor origin, per 100 dystrophin-positive myofibers 21 days after engraftment of 10,000 satellite cells isolated from Pax3 GFP/+ (wt) and Pax3 GFP/+ ; Fmr1 −/− (Fmr1 −/−) mice. Values indicate mean (n ≥ 3) ± s.e.m. **p < 0.01. ns not significant

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