Fig. 1

Protection from myofiber damage and myonuclear apoptosis in MKP-5-deficient mice in response to injury. Representative image of sections from the soleus muscle of mkp-5 ^+/+ and mkp-5 ^−/− mice at 10 days after cardiotoxin (CTX)-induced injury (a) Representative image of Evans blue dye uptake in the soleus muscle of mkp-5 ^+/+ and mkp-5 ^−/− mice at 10 days after CTX-induced injury. The graph (right panel) represents quantitation showing the percentage of Evans blue dye area in the soleus section in each genotype. Scale bar: 100 μm. (b) Stained with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to detect for apoptotic nuclei (arrows). Myofibers were stained with dystrophin (red) and nuclei stained with DAPI (blue). Nuclei either within or on the dystrophin boundaries of the myofiber were defined as myonuclei and quantitated. The graph (right panel) represents quantitation showing the TUNEL-positive myonuclei in each genotype. Scale bar: 20μm