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Fig. 4 | Skeletal Muscle

Fig. 4

From: Loss of MKP-5 promotes myofiber survival by activating STAT3/Bcl-2 signaling during regenerative myogenesis

Fig. 4

MKP-5 deficiency in regenerating skeletal muscle reduces oxidative damage. (a) Hydrogen peroxide release from isolated mitochondria derived from soleus muscle of uninjured and 10 days after CTX-induced injury of mkp-5 +/+ and mkp-5 −/− mice. 4-HNE-conjugated protein content in soleus muscle was determined by immunoblotting using anti-4-HNE antibodies in (b) uninjured skeletal muscle and (c) 10 days after CTX-induced injury of skeletal muscle. The graphs below represent the relative intensity of 4-HNE-conjugated proteins quantitated by densitometric analysis of equal lane areas normalized to Erk1/2 levels. Catalase expression in soleus muscle was determined using anti-catalase antibodies in (d) uninjured and (e) 10 days after CTX-induced injury. The graphs represent quantitation of catalase expression normalized to Erk1/2. Values are means ± standard errors of the means (SEM). **P < 0.01, n = 5 per genotype

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