Fig. 6

Increased JAK1/2 and STAT3 signaling in regenerating skeletal muscle of MKP-5-deficient mice. Phosphorylation levels of JAK1 (Y1022 /1023) and JAK2 (Y1007) in soleus muscle of (a) uninjured and (b) 10 days after CTX-induced injury, in mkp-5 ^+/+ and mkp-5 ^−/− mice were determined by immunoblotting with phospho-JAK and total JAK antibodies. Graphs represent the quantitation of immunoblots showing phosphorylated JAK1/2 normalized to JAK1/2 from uninjured and injured skeletal muscle of each genotype. Phosphorylation levels of STAT3 (Y705 and S727) in soleus muscle were determined in (c) uninjured and (d) 10 days after CTX-induced injury in mkp-5 ^+/+ and mkp-5 ^−/− mice. The graphs represent quantitation of phosphorylated STAT3 normalized to STAT3. Values are means ± standard errors of the means (SEM). *P < 0.05, **P < 0.01, n = 5 per genotype