Fig. 7

Activation of p38 MAPK and JNK induce the phosphorylation of STAT3 (S727) and Bcl-2 expression. C2C12 myoblasts were transfected with either vector control, constitutively active mutants of MKK6(EE) or MKK7(DD). After 24 h, myoblasts were induced to undergo differentiation for 48 h. (a) Representative images of differentiated C2C12 myoblasts for 48 h. (b) Transfected differentiated C2C12 myotubes were lysed and immunoblotting for the expression of the indicated proteins. Graph showing quantitation of (c) phosphorylation levels of STAT3 (S727) and (d) Bcl-2 protein expression. The graphs represent quantitation of phosphorylated STAT3 normalized to STAT3, and quantitation of Bcl-2 proteins normalized to SHP-2. Values are means ± standard errors of the means (SEM) and were analyzed by ANOVA with Tukey’s multiple comparison test. Two asterisks indicate MKK6(EE) significantly different vs. vector (P < 0.01). Two plus signs indicate MKK7(DD) significantly different vs vector (P < 0.01)