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Fig. 1 | Skeletal Muscle

Fig. 1

From: HGF potentiates extracellular matrix-driven migration of human myoblasts: involvement of matrix metalloproteinases and MAPK/ERK pathway

Fig. 1

Phenotypic features of the CHQ human myoblast preparation. a Culture purity was determined by cytofluorometric detection of CD56, a typical myoblast surface marker. As seen in the FACS profile, 87% of this cell preparation did correspond to myoblasts, since labeling was positive for the anti-CD56 antibody, as compared to the black curves, generated after binding of an unrelated antibody. b The myogenicity of the cells was confirmed by desmin expression in immunofluorescence, using an anti-desmin monoclonal antibody. c Immunodetection of c-met by western blotting of whole-cell extracts. Hepatoma cell line HEP-G2: positive control. d Flow cytometry profiles of the expression of CD29 (β1-integrin chain), CD49d, CD49e (integrin α-chains of the fibronectin receptors VLA4 and VLA5 respectively), and CD49f (integrin α-chain of the laminin receptor VLA6). VLA-7 expression was detected by immunostaining using an anti-CD49g antibody. Data are representative of three independent experiments

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