Fig. 2

HGF treatment does not affect the adherence of human CHQ myoblast onto the extracellular matrix. Panel a show the increased number of adherent human CHQ myoblasts cells on laminin and fibronectin substrates, in the absence or presence of HGF, as a function of time (15 min; 30 min; 1 h; 2 h). Adhesion was not enhanced in the presence of HGF. b Representative images of primary CHQ myoblasts cultured on ECM-coated (LN-111 or FN) surfaces in the absence or presence of HGF visualized by phalloidin alexa-488 staining and analyzed by fluorescence microscopy. Data of three independent experiments of 15 min and 2 h (200×). (Magnification bar: 100 μm). BSA: Bovine serum albumin; GT: gelatin; LN: Laminin-111; FN: Fibronectin. Bars represent a mean ± SD. Results correspond to three independent experiments. ***p < 0.001 versus BSA