Fig. 3
From: Utrophin influences mitochondrial pathology and oxidative stress in dystrophic muscle
Assessment of markers of oxidative capacity in C57BL/10, mdx, dko and mdx-Fiona mice. Representative images of extensor digitorum longos muscles from C57BL/10 (C57), mdx, dko and mdx-Fiona (mdx-Fio) mice assessed for (a) mitochondrial aggregation (Via Gomori’s trichrome; GT), (b) cytochrome oxidase (COX) activity and (c) succinate dehydrogenase (SDH) activity indicated by increased intensity of red, brown and blue staining, respectively (n = 5–6/group). Enzyme activity of mitochondria respiratory chain complexes (d) I and (e) II (n = 4/group). f Representative images and quantification of protein expression in mitochondria respiratory chain complexes I–V in quadriceps muscles from C57, mdx, dko and mdx-Fio mice, normalised to loading control (total protein stain (TPS)). Data are represented as mean ± SEM, no differences were observed between groups. Representative image n = 3/group, quantification generated from n = 9/group