Fig. 12
From: The breaking and making of healthy adult human skeletal muscle in vivo
Myogenic and inflammatory cell activity viewed in serial cross sections. Microscope images of serial sections of one biopsy collected from regenerating human muscle 7 days after an injury protocol. Sixty serial sections (12 μm thick) were cut and stained for various markers. Shown here is a selection of these sections, where the section number (number sign) and proteins stained are indicated. Note the change in fibre diameter of the injured fibres, which are most clearly seen in section #19 (asterisk) by their lack of dystrophin (dys) staining and preserved basement membrane (collagen IV, col4). As observed in the single fibre analysis, these fibres are characterised by infiltration of macrophages (CD68+), the presence of neonatal/embryonic myosin (MHCne) and differentiating myogenic cells (desmin+, CD56+, myogenin+, nestin+). Scale bar 100 μm