Fig. 6
From: The breaking and making of healthy adult human skeletal muscle in vivo
Lack of striations in necrotic zones. a. Low magnification confocal microscope image of a regenerating human muscle fibre 7 days post injury (and an uninjured fibre above it), stained for desmin (green), actin (phalloidin, red) and nuclei (blue). Note the distinct necrotic (asterisk) and regenerating (arrow) zones. Scale bar, 100 μm. The area indicated by the dashed line box was imaged at higher magnification, shown in b. The image slice (10) through the middle of the regenerating zone reveals a cell-dense area lacking actin striations. A desmin-positive sheath surrounds this region (slices 15–17). The dashed lines indicate the location of the two YZ orthogonal views. Note the wider necrotic zone devoid of cells and striations in its core. Scale bars, 20 μm