Fig. 7

Direct comparison of fixed and unfixed satellite cells identify immediate response genes not present the in vivo state. a Boxplots of four examples of genes found commonly upregulated in QSCs in the different datasets showing the distribution of intensities values in QSCs and ASCs. Colored dots indicate each dataset. Shape of the dot indicates whether the gene is significantly differentially expressed or not. b Fold change of mRNA (log10) between 0 h + PFA and 5 h + PFA. Blue bars indicate a higher expression in 0 h + PFA condition; the red bars indicate a higher expression in 5 h + PFA condition. Color intensities are proportional to the fold change. c c-Jun, Jun B, and Jun D protein levels from satellite cells at 0, 5, 10, 15 h after isolation (with and without PFA treatment) were measured by Western blotting, and band intensities were quantified by densitometric analysis with the ImageLab software (right). Basal levels of c-Jun, Jun B, and Jun D mRNA from satellite cells at 0, 5, 10, 15 h after isolation (with and without PFA treatment) were measured by real-time PCR (left)