Table 1 Summary of analyzed transcriptomic datasets of activated and quiescent states of mouse muscle stem (satellite) cells. Three new high-throughput experimental setups and six publically available microarray datasets comparing activated satellite cells (ASCs) and quiescent satellite cells (QSCs) are shown in the rows. The biological, experimental, and technical details of each experiment are shown in the different columns of the Table

Ref/code

Quiescent [high/low] D3 Activated [high/low]

Quiescent activated

Fetal R26^NICD [E17.5/E14.5]

GSE47177 Liu et al. [9]

GSE3483 Fukada et al. [10]

GSE15155 Pallafacchina et al. [11]

GSE38870 Farina et al. [12]

GSE70376 García-Prat et al. [13]

GSE81096 Lukjanenko et al. [14]

Num. of samples

3 QSC_Pax7 low, 3 ASC_Pax7 low, 3 QSC_Pax7 high, 3 ASC_Pax7 high

3 QSC, 3 P8_ASC

3 "QSC," 3 "ASC

3 QSC,

3 ASC 60 h,

3 ASC 84 h

3 QSC, 3 ASC

3 QSC, 3 ASC

3 QSC, 3 ASC

4 QSC, 4 ASC

6 QSC, 5 ASC

Date

2013

2007

2015

2013

2007

2010

2012

2015

2016

Anatomy

Tibialis anterior

Limb, body wall, diaphragm

Forelimbs

Hindlimb

Hindlimb

Diaphragm, pectoralis, abdominal muscles

Tibialis anterior

Tibialis anterior

Tibialis anterior, gastro-necmius, quadriceps

Sex

M

M, F

M

F

M, F

F

M

M

Age

6–8 w

P8, 4–5 w

E14.5, E17.5

8 w

8–12 w

6 w

3–6 m

Young: 3 m, old: 20–24 m

Young: 9–15 w, old: 20–24 m

Strain

C57BL/6

B6.129

C57BL/6 (Jackson)

C57BL/7 (nihon clea)

C57BL/6 x DBA2 (??)

C57BL/6

C57BL/6 (Janvier)

Reporter

Tg:Pax7-nGFP (10% high, 10% low)

Pax7^nGFP/+

Myf5^Cre+: R26^{stop-NICDgfp/+}

Pax7^CreER/+ R26^eYFP/+

Pax3^GFP/+ (high GFP)

Activation

Notexin

P8 = “activated”

E14.5 = “activated”

BaCl₂

QSCs in culture for 4 d

Pax3^GFP/+, Pax3^GFP/+:mdx:mdx

Adult; adult mdx; 1 w old; 3 d in culture

Injury: BaCl₂ (50 μL 1.2%)

Cardiotoxin

Cardiotoxin

QSCs Purif.

Reporter

Reporter

Reporter

FACS: Pax7^CreER/+; R26^eYFP/+

FACS: CD45−/SM/C-2.6+

Reporter

FACS: syndecan-3

FACS: integrin-alpha7+/Lin−/CD31−/CD45−/CD11b−/Sca1-

CD34+/integrin-alpha7+/Lin-

Timing

Quiescent and 3 d postinjury

E17.5(“Q”), E14.5(“A”)

36 h (1.5 d), 60 h (2.5 d), 84 h (3.5 d) postinjury

4 d in culture

3 d in culture

12 h or 48 h postinjury

72 h (3 d)

72 h (3 d)

Platform

Affymetrix Mouse Gene 1.0ST

Affymetrix 430_2.0

Affymetrix Mouse Gene 1.0ST, Affymetrix Mouse Gene 2.0ST

Affymetrix Mouse Gene 1.0ST

Affymetrix 430A

Affymetrix 430_2.0

Affymetrix 430_2.0

Agilent 028005 SurePrint G3Mouse 8x60k Microarray

Illumina MouseRef-8 v2.0