Table 1 Primer design for the RT-PCR assay
From: Low-intensity pulsed ultrasound prevents muscle atrophy induced by type 1 diabetes in rats
Gene | Primer | PCR product length (bp) | Sequence 5′ to 3′ | Tm (°C) |
|---|---|---|---|---|
MSTN | F | 423 | GATTATCACGCTACCACG | 58.9 |
R | ATTCAGCCCATCTTCTCC | |||
ActRIIB | F | 163 | GCAGTCGTGGCAGAGTGAGCG | 51.7 |
R | CTTGAGGTAATCCGTGAGGGAGC | |||
Akt | F | 269 | TAGGCATCCCTTCCTTACAG | 55.8 |
R | GCCCGAAGTCCGTTATCT | |||
FoxO1 | F | 459 | TCAAGGATAAGGGCGACAG | 54.4 |
R | GTGGATACACCAGGGAATG | |||
mTOR | F | 368 | CTGGCTTCCAACCCTAAA | 53.5 |
R | TCTCCAAATCCCACTCCC | |||
GLUT4 | F | 545 | GTC ATC AAC GCC CCA CAG | 56.8 |
R | TCA GGA CAG AAG GGC AAC AG | |||
GAPDH | F | 292 | CGACTGTTAGAACTCCCTCA | 57.4 |
R | CATTGGGGGTAGGAACAC |