Fig. 4

Expression levels of muscle-specific miRNAs and TGF-β in the regenerating gastrocnemius muscles from CAPN3-KO (KO) and wild type (WT) at 4 and 12 weeks post CTX injection. mirR-206 (a), miR-1(b), miR-133a (c), and TGF-β (d) levels in the regenerating CAPN3-KO and WT are relative to their baseline levels (dashed line), obtained from the uninjected muscles of 4 weeks post injection cohorts in each group. Expression levels were assessed by qPCR. For miRNAs, internal control was U6 snRNA, and for TGF-β, it was GAPDH. *P < 0.05, **P < 0.01 by two-tailed t test. Error bars represent ± SEM; n = 3–4 in WT and n = 4–5 in KO