Fig. 6

Analysis of mitochondrial content and the expression of mitochondrial biogenesis markers in the regenerating gastrocnemius muscles from CAPN3-KO (KO) and wild type (WT) at 4 and 12 weeks post CTX injection. Mitochondria content (mtDNA/nDNA) of regenerating muscles (a) are expressed as percent of their baseline levels (dashed line), obtained from the uninjected muscles of 4 weeks post injection cohorts in each group. The expression levels of PGC1α (b) and ATP5D (c) in the regenerating muscles from CAPN3-KO and WT are relative to their baseline levels (dashed lines) in the uninjected contralateral muscles of 4 weeks post injection cohorts in each group. Expression levels were determined by qPCR. PGC1α and ATP5D expressions were normalized to GAPDH expression. *P < 0.05, **P < 0.01 by two-tailed t test. Error bars represent ± SEM; n = 3–4 in WT and n = 4–5 in KO