Fig. 7

Representative western blot images and analysis of AMPK signaling in CAPN3-KO (KO) and wild type (WT) gastrocnemius muscles. Expression of P-AMPK (Thr¹⁷²), P-LKB1 (Ser⁴²⁸), P-Raptor (Ser⁷⁹²), and P-Ulk1 (Ser⁵⁵⁵) at 4 weeks (a) and 12 weeks (b) post CTX injection. The results show expression levels of the phosphorylated form of proteins normalized to non-phosphorylated forms. *P < 0.05, **P < 0.01 two-tailed t test. Error bars represent ± SEM; n = 3–4 in WT and n = 4–5 in KO. Coomassie Blue stained membranes were shown as representative loading controls