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Fig. 4 | Skeletal Muscle

Fig. 4

From: Development of the excitation-contraction coupling machinery and its relation to myofibrillogenesis in human iPSC-derived skeletal myocytes

Fig. 4

Percentage of α-actinin, RyR1, and Cav1.1 positive cells during differentiation. Symbols represent mean ± SEM of the percentage of α-actinin+/RyR1+/Cav1.1+ cells as counted from immunofluorescent microphotographs of iPSC-derived skeletal myocytes at different stages of their differentiation in culture. The total number of cells was determined by counting all Hoechst-stained nuclei on the microphotograph. Immunofluorescent cells were counted from four to ten frames per independent experiment (×60 magnification); three independent experiments were performed at each time point for each type of protein (labeled separately). Note that the percentage of immunolabeled cells for the three proteins reaches a stable level after the second week of differentiation

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Skeletal Muscle

ISSN: 2044-5040

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