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Table 1 Changes of the intracellular Ca2+ signal characteristics with cell differentiation

From: Development of the excitation-contraction coupling machinery and its relation to myofibrillogenesis in human iPSC-derived skeletal myocytes

Days

n (N)

Integrated fluorescence (% of baseline)

Peak (% baseline)

Time to peak

(s)

Single decay, Ï„ (s)

Double decay (s)

Contraction (% of Cells)

Ï„ fast

Ï„ slow

3

24 (3)

9.1 ± 8.0

***

NA

NA

NA

NA

NA

0

4

28 (4)

129.7 ± 26.6

***

13.9 ± 1.4

***

NA

NA

NA

NA

0

5

24 (3)

411.7 ± 28.7

***

32.6 ± 1.7

***

9.6 ± 1.0

9.7 ± 1.1

NA

NA

0

7

42 (6)

539.6 ± 27.6

***

44.6 ± 2.0

*

11.5 ± 0.9

8.4 ± 0.7

NA

NA

~ 25

Mature

107 (7)

857.2 ± 23.2

51.3 ± 1.3

12.2 ± 0.3

NA

9.1 ± 0.5

53.4 ± 3.6

~ 100

  1. Statistical significance is indicated below the mean values: ***p < 0.001; **p < 0.01, *p < 0.5
  2. NA not applicable, n total number of cells, N number of independent experiments performed
  3. All numbers represent mean values. Results obtained during the first week of human iPSC-derived myogenic progenitors differentiation are compared with those of mature skeletal myocytes