Fig. 1

DUX4c overexpression induces misdistribution of troponin T and abnormal distribution of myonuclei. Healthy human primary myotubes were transfected with pCIneo-DUX4c or the empty pCIneo expression vector and switched to differentiation medium. a Five days later, troponin T was detected by immunofluorescence (green), and nuclei were labeled with DAPI (blue). Scale bar: 20 μM. b A 20 μg sample of total protein extracted from the same cells was separated via electrophoresis (PAGE-SDS), then transferred to a Western blot, immunodetected with the indicated primary antibodies and appropriate secondary antibodies coupled to HRP, and revealed through chemiluminescence