Fig. 4

dFSHD myotubes present abnormal clusters of nuclei overexpressing DUX4c as well as cytoplasmic DUX4c and α-tubulin disorganization. Healthy and FSHD primary myoblasts were differentiated, then fixed 6 days later, and DUX4c (red) and α-tubulin (green) were detected by immunofluorescence. A large cluster of myonuclei (> 16, indicated by *) can be observed, while such clusters were very seldom found in healthy cultures. Scale bars: 100 μm