Fig. 3

Nilotinib late treatment impairs terminal differentiation. a Outline of C2C12 skeletal muscle differentiation and late Nilotinib treatment protocol. Nilo, Nilotinib. b Total protein content was determined using the BCA assay and O.D. obtained in control of Nilotinib-treated cells. n = 3; ***P < 0.001; DMSO vs Nilotinib; with two-tailed Student’s t test. c Representative crystal violet staining images. Insets show a higher magnification of D6 myotubes. Scale bars: 500 μm. d Western blot analysis of three representative experiments to evaluate the total amount of MyHC. Tubulin was used as the loading control. e Quantification of three independent experiments to evaluate MyHC expression. Values correspond to the mean ± SEM. *P < 0.05; n = 3; with two-tailed Student’s t test