Fig. 2
From: A novel in vitro model for the assessment of postnatal myonuclear accretion
Increased in vitro postnatal myonuclear accretion in C2C12 cells upon IGF-I treatment. a-c Staining-based assessment of myonuclear accretion 2 days after initiation of co-culturing +/- 10 nM IGF-I. a total number of myotubes, b number of hybrid myotubes, c % hybrid myotubes. d-f Luciferase-based assessment of myonuclear accretion 3 days after initiation of co-culturing +/− 10 nM IGF-I. D) luciferase activity (RLU) per well, E) protein content (μg/μL) per well, F) relative luciferase activity (RLU/protein content) per well. Values are means ± SEM, n = 4. *p < 0.05, **p < 0.01, ***p < 0.001