Fig. 3
From: A novel in vitro model for the assessment of postnatal myonuclear accretion
Decreased in vitro postnatal myonuclear accretion in C2C12 cells upon CytoD, MSTN and TNFα treatment. Luciferase-based assessment of myonuclear accretion 3 days after initiation of co-culturing +/- 0.3 μM CytoD (A-C), 250 ng/mL MSTN (D-F), or 10 ng/mL TNFα (G-I). a Luciferase activity (RLU) per well, b protein content (μg/μL) per well, c relative luciferase activity (RLU/protein content) per well, d luciferase activity (RLU) per well, e protein content (μg/μL) per well, f relative luciferase activity (RLU/protein content) per well, g luciferase activity (RLU) per well, h protein content (μg/μL) per well, i relative luciferase activity (RLU/protein content) per well. Values are means ± SEM, n = 4. *p < 0.05, **p < 0.01, ***p < 0.001