Fig. 5
From: A novel in vitro model for the assessment of postnatal myonuclear accretion
Increased in vitro postnatal myonuclear accretion in C2C12 cells upon recovery from myotube damage and atrophy. Luciferase-based assessment of myonuclear accretion 3 days after initiation of co-culturing of myotubes pre-treated by 1-h electrical pulse stimulation, with myoblasts in presence of conditioned medium (n = 6). a Luciferase activity (RLU) per well, b protein content (μg/μL) per well, c relative luciferase activity (RLU/protein content) per well. Luciferase-based assessment of myonuclear accretion 3 days after initiation of co-culturing of myotubes pre-treated +/- 15 μM LY (24 h pre), with myoblasts in presence or absence of 15 μM LY (72 h post) (n = 4). d luciferase activity (RLU) per well, e protein content (μg/μL) per well, f relative luciferase activity (RLU/protein content) per well. T = initiation and duration of treatment (h) relative to start of co-culturing. Values are means ± SEM, n = 4. *p < 0.05, **p < 0.01, ***p < 0.001 compared to control or between indicated groups