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Fig. 3 | Skeletal Muscle

Fig. 3

From: HDAC4 preserves skeletal muscle structure following long-term denervation by mediating distinct cellular responses

Fig. 3

HDAC4 mediates the activation of autophagy in skeletal muscle upon denervation. a Real-time PCR for autophagic markers in control (CTR) and HDAC4mKO skeletal muscle, 1 week following denervation. Data are shown as mean ± SEM; n = 8; two-way ANOVA revealed an interaction (F = 29.3; df 1; p = 0.001 for Atg7; F = 28.5; df 1; p = 0.001 for Gabarapl1; F = 9.65; df 1; p = 0.004 for p62); **p < 0.01 by Tukey’s HSD test. b Representative western blot for LC3b and p62 proteins in control (CTR) and HDAC4mKO TA muscles, 1 week following denervation, in the absence (−) or after colchicine (CL) treatment. Gapdh was used as loading control. c Densitometric analyses for LC3b and p62 proteins, 1 week following denervation, in the absence (−) or after colchicine (CL) treatment. Data are shown as mean ± SEM; n = 3–4; two-way ANOVA revealed an interaction (F = 114; df 1; p = 0.001 for LC3b; F = 14.29; df 1; p = 0.036 for p62); *p < 0.05 versus CTR Con; $p < 0.05 versus CTR Den by Tukey’s HSD test. d Representative western blot for LC3b and p62 proteins, 2 weeks following denervation. Gapdh was used as loading control. e Densitometric measurements for LC3b and p62 proteins, 2 weeks following denervation. n = 3–4; two-way ANOVA revealed an interaction (F = 13.21; df 1; p = 0.046 for LC3b; F = 7.35; df 1; p = 0.01 for p62); *p < 0.05 versus CTR Con by Tukey’s HSD test. f Representative western blot analyses for LC3b and p62, 4 weeks after denervation. Gapdh was used as loading control. g Densitometric measurements for LC3b and p62 proteins, 4 weeks following denervation. n = 4; two-way ANOVA revealed an effect of denervation (F = 6.34; df 1; p = 0.028 for LC3b; F = 10.68; df 1; p = 0.0061 for p62)

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