Fig. 5

HDAC4 mediates the activation of OS response in skeletal muscle upon denervation. a DHE staining of control (CTR) and HDAC4mKO TA muscles following 1 week of denervation and relative quantification. Scale bar = 50 μm. n = 5; two-way ANOVA showed an interaction (F = 8.74; df 1; p = 0.01); *p < 0.05 by Tukey’s HSD test. b Densitometric analysis of western blots for Gp91phox in CTR and HDAC4mKO TA muscles, 1 week after denervation. n = 4; two-way ANOVA showed an interaction (F = 20.14; df 1; p = 0.0004); *p < 0.05 by Tukey’s HSD test. c Quantification of ROS and RNS 1 week of denervation. n = 4–5; two-way ANOVA revealed an effect of denervation (F = 9; df 1; p = 0.009) and an interaction (F = 5; df 1; p = 0.041); *p < 0.05 by Tukey’s HSD test. d Real-time PCR of antioxidant enzymes in CTR and HDAC4mKO skeletal muscles, 1 week following denervation. n = 4–5; two-way ANOVA revealed an interaction (F = 8.82; df 1; p = 0.0082 for GSR; F = 5.24; df 1; p = 0.03 for GST; F = 14.07; df 1; p = 0.0015 for NQO1 and F = 6.14; df 1; p = 0.02 for catalase); *p < 0.05; **p < 0.01 by Tukey’s HSD test. e Activity of GSR and GST, 1 week following denervation. n = 5–6; two-way ANOVA showed an interaction (F = 11.16; df 1; p = 0.0026 for GSR; F = 6.16; df 1; p = 0.026 for GST); *p < 0.05 by Tukey’s HSD test. f DHE staining of CTR and HDAC4mKO TA muscles following 2 weeks of denervation. Scale bar = 50 μm. g Densitometric analysis of western blots for Gp91phox in CTR and HDAC4mKO TA muscles, 2 weeks after denervation. n = 4; two-way ANOVA showed an interaction (F = 36.77; df 1; p = 0.0001); *p < 0.05 by Tukey’s HSD test. h DHE staining of CTR and HDAC4mKO TA muscles following 4 weeks of denervation. Scale bar = 50 μm. i Densitometric analysis of western blots for Gp91phox in CTR and HDAC4mKO TA muscles, 4 weeks after denervation. n = 4; two-way ANOVA revealed an effect of denervation (F = 14.97; df 1; p = 0.0026)