Fig. 2

Effect of training and Parkin on whole muscle protein markers. a Representative Western blot of Parkin expression in untrained and trained skeletal muscle of control wild-type (WT) mice above. A graphical representation is shown below (n = 5, *P < 0.05, vs UT). b Representative immunoblots of COXI, COXII, COXIV and TFAM expression in untrained and trained skeletal muscle of Parkin KO and WT animals. c A graphical representation of whole muscle COXI shown (n = 4, *P < 0.05, main effect of training; #P < 0.05, vs trained WT). d Quantification of whole muscle COXII (n = 6, *P < 0.05, main effect of training). e Whole muscle COXIV expression (n = 4, *P < 0.05, main effect of training, #P < 0.05, main effect of genotype). f Quantification of whole muscle TFAM (n = 4, *P < 0.05, main effect of training). Values are means ± SEM. †P < 0.05, interaction effect of training and genotype. α-tubulin was used for muscle loading control. WT, wild type; KO, Parkin knockout; UT, untrained; T, trained; COXI, cytochrome c oxidase subunit 1; COXII, cytochrome c oxidase subunit 2; COXIV, cytochrome c oxidase subunit 4; TFAM, mitochondrial transcription factor A; A.U., arbitrary units