Fig. 3

Effect of training and Parkin on key regulators of mitochondrial biogenesis. a Representative Western blots of PGC-1α, PARIS and p-ACC expression in untrained and trained skeletal muscle of Parkin KO and WT animals. b A graphical representation of whole muscle PGC-1α (n = 4, *P < 0.05, vs untrained WT). c Quantification of whole muscle PARIS (n = 6, *P < 0.05, main effect of training; #P < 0.05, vs untrained WT). d A graphical representation of whole muscle phosphorylation of ACC (n = 6, *P < 0.05, main effect of training; #P < 0.05, vs untrained KO). Values are means ± SEM. †P < 0.05, interaction effect of training and genotype. α-tubulin was used for muscle loading control. WT, wild type; KO, Parkin knockout; UT, untrained; T, trained; p-ACC, phospho-acetyl-CoA carboxylase; PARIS, Parkin-interacting substrate; PGC-1α, peroxisome proliferator gamma coactivator-1α; A.U., arbitrary units