Fig. 7
From: Role of Parkin and endurance training on mitochondrial turnover in skeletal muscle
Mitophagic p62 flux following acute exercise, training, and combined treatments in WT and KO animals. Representative Western blots of mitochondrial p62 localization of WT (a) and Parkin KO (b) mice injected with water (Veh) or 0.4 mg/kg colchicine (Col). Quantification of mitochondrial p62 flux (c) is shown (n = 5). Mitophagic p62 flux was assessed under basal conditions, and immediately following acute exercise, in untrained and trained groups of WT and KO animals. The fold change in p62 flux (d) was calculated with acute exercise-induced values over basal values (n = 5). Values are means ± SEM. *P < 0.05, main effect of genotype; #P < 0.05, main effect of exercise. Voltage-dependent anion channel (VDAC) was used as a mitochondrial loading control. WT, wild type; KO, Parkin knockout; UT, untrained; T, trained; p62, sequestosome 1; A.U., arbitrary units