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Table 4 Histological analyses for skeletal muscles and serum creatinine kinase levels among groups of P448Lneo (FKRP) without and with treadmill exercise (FKRP-treadmill) and control (BL6) mice at 6 months of age

From: Skeletal, cardiac, and respiratory muscle function and histopathology in the P448Lneo− mouse model of FKRP-deficient muscular dystrophy

Measurement BL6 control FKRP FKRP-treadmill Significantly different groups (adjusted p values using ANOVA followed by post hoc analysis)
N Mean ± SD N Mean ± SD N Mean ± SD
Inflammation
(foci/mm2)
GAS 8 0.03 ± 0.03 8 0.5 ± 0.4 12 0.9 ± 0.3 #BL6 vs FKRP-treadmill: p < 0.0001
Quad 8 0.05 ± 0.04 8 0.6 ± 0.2 12 1.1 ± 0.5 #BL6 vs FKRP: p < 0.01,
#BL6 vs FKRP-treadmill: p < 0.0001
Triceps 8 0.04 ± 0.06 8 0.6 ± 0.4 12 1.1 ± 0.3 #BL6 vs FKRP: p < 0.01,
#BL6 vs FKRP-treadmill: p < 0.0001
% fibrosis Quad 8 0.29 ± 0.07 8 0.41 ± 0.18 12 0.61 ± 0.15 BL6 vs FKRP-treadmill: p < 0.001,
FKRP vs FKRP-treadmill: p < 0.05
  GAS 3 0.47 ± 0.23 3 46.18 ± 5.4 3 38.44 ± 5.1 NP
% central nucleation Quad 3 0.2 ± 0.3 3 56.0 ± 1.9 3 51.1 ± 2.1 NP
  Triceps 3 1.44 ± 1.11 3 65.71 ± 1.1 3 75.16 ± 8.4 NP
  GAS 3 34.27 ± 0.7 3 37.9 ± 3.6 3 42.7 ± 2.6 NP
Fiber diameter size (μm) Quad 3 48.2 ± 5.5 3 44.8 ± 1.2 3 49.7 ± 1.6 NP
  Triceps 3 37.75 ± 1.2 3 37.03 ± 1.8 3 43.70 ± 2.0 NP
  GAS 3 0.5 ± 0.2 3 7.6 ± 5.4 3 10.7 ± 5.1 NP
SD of % central nucleation Quad 3 0.4 ± 0.5 3 8.9 ± 3.9 3 12.6 ± 4.4 NP
  Triceps 3 1.28 ± 1.1 3 15.94 ± 1.1 3 8.17 ± 8.4 NP
  GAS 3 1.93 ± 0.7 3 8.5 ± 3.6 3 8.73 ± 2.6 NP
SD of fiber size Quad 3 11.8 ± 1.3 3 20.5 ± 1.8 3 21.5 ± 1.9 NP
  Triceps 3 1.93 ± 1.2 3 4.8 ± 1.8 3 6.96 ± 2.0 NP
Serum creatinine kinase(μ/l) 8 254 ± 131 7 749 ± 405 12 947 ± 575 BL6 VS. FKRP-treadmill: p < 0.05
  1. #Kruskal-Wallis test followed by Dunn’s multiple comparison test used. Statistical measures not performed on measures with N = 3. GAS gastrocnemius, Quad quadriceps, SD standard deviation, NP not performed