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Fig. 2 | Skeletal Muscle

Fig. 2

From: An RNAi based screen in Drosophila larvae identifies fascin as a regulator of myoblast fusion and myotendinous junction structure

Fig. 2

Fascin is localized to actin and the nucleus in muscle. a Images from different focal planes of dissected L3 larvae showing that fascin (sn) is colocalized with both Phalloidin (F-actin) (left two panels) and Hoechst (nuclei) (right two panels). b Immunofluorescence images from stage 16 embryos stained for Tropomyosin to identify the muscles (magenta) and the nuclei in the LT muscles (green) in control and sn28 mutant embryos. Scale bar, 10 μm. c Graph indicating the distance between the dorsal end of the muscle and the nearest nucleus in control and sn28 mutant embryos. d Graph indicating the distance between the ventral end of the muscle and the nearest nucleus in control and sn28 mutant embryos. e, f Immunofluorescence images from stage 16 embryos stained for Tropomyosin to indicate the muscles (magenta) and the nuclei within the LT muscles (green) in embryos where RNAi against either mCherry (control) or fascin (sn RNAi) was driven by Twist-GAL4 (e) or Dmef2-GAL4 (f). g, h Graphs indicating the average distance between the dorsal end of the muscle and the nearest nucleus (g) or the ventral end of the muscle and the nearest nucleus (h) in embryos of indicated genotypes. All data (c, d, g, h) were compared to their control by Student’s t test. **p < 0.01

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