Fig. 3
Fascin is necessary for myoblast fusion. a Immunofluorescence images of stage 16 embryos stained for Tropomyosin to indicate the muscles (magenta). Images are focused on the lateral transverse (LT) muscles in stage 16 embryos. Green arrowheads indicate unfused myoblasts. Scale bar, 10 μm. b Graph indicating the percentage of embryos that had at least one hemisegment with > 4 LT muscles (Extra LTs) or < 4 LT muscles (missing LTs). Values that exceed 100% indicate that some embryos had one hemisegment with > 4 LT muscles (extra muscles) and another hemisegment with < 4 LT muscles (missing muscles). c Graph indicating the number of apRed positive nuclei per hemisegment. Student’s t test was used for comparison to controls. ****p < 0.0001. d Graph indicating how frequently different numbers of unfused myoblasts are seen in control (black) and sn28 mutant embryos (green). e Graph indicating the viability of the sn28 mutant animals (green) compared to controls (black)