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Fig. 4 | Skeletal Muscle

Fig. 4

From: An RNAi based screen in Drosophila larvae identifies fascin as a regulator of myoblast fusion and myotendinous junction structure

Fig. 4

Fascin has muscle autonomous effects on myoblast fusion. a Stage 16 embryos stained for tropomyosin to identify the muscles (magenta). Embryos expressed RNAi against fascin under the control of Twist-GAL4 (top) or Dmef2-GAL4 (bottom). Green arrowheads indicate unfused myoblasts. Scale bar, 10 μm. b Graph indicating the percentage of embryos of indicated genotypes that have at least one hemisegment with either > 4 LT (extra LTs) muscles or < 4 LT muscles (missing LTs). c Graph indicating the number of apRed positive nuclei incorporated into LT muscles per hemisegment in indicated genotypes. Student’s t test was used for comparison to controls. *p < 0.05. d Graph indicating how frequently different numbers of unfused myoblasts were seen in indicated genotypes. e Graph indicating the viability of animals with indicated genotypes

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