Fig. 5
Genetic disruption of fascin did not affect larval muscle structure. a Immunofluorescence images of the third ventral longitudinal muscle (VL3) in L3 larvae of indicated genotypes. Sarcomeres (magenta) were stained with Phalloidin to identify the muscle and Hoechst (green) was used to identify the nuclei. Scale bar, 25 μm. b Graph indicating the actual internuclear distance divided by the maximal internuclear distance in indicated genotypes. c Graph indicating the area of the muscles as a proxy for muscle size in the indicated genotypes. d Graph indicating the number of nuclei in indicated genotypes. All data (b–c) were compared to their control by Student’s t test. All differences were statistically insignificant