Fig. 7
Fascin is necessary for proper myotendinous junction organization a Immunofluorescence images of the MTJ of muscle DO2 stained for tropomyosin and βPS-integrin in control (top) and sn28 mutant embryos (bottom). Scale bar, 10 μm. b Representative intensity profile of the βPS-integrin signal in control (black) and sn28 mutant embryos (green). c Graph indicating the width of the βPS-integrin signal defined by the points at which the signal is 25% of the maximum. d, e Immunofluorescence images of the MTJ of muscle DO2 stained for tropomyosin and βPS-integrin in animals in which twist-GAL4 was used (d) or DMef2-GAL4 was used (e) to express RNAi against either mCherry (control) or fascin (sn RNAi). f, g Representative intensity profiles of the βPS-integrin signal in indicated genotypes. h Graph indicating the width of the βPS-integrin signal in indicated genotypes as defined by the points at which the signal is 25% of the maximum. All data (c, h) were compared to their control by Student’s t test. **p < 0.01; ****p < 0.0001