Fig. 5
CTX-induced muscle injury. Mice were injected with 10 μL of 10 μM CTX in one FDB and 10 μL sterile PBS in the contralateral FDB. Mice were euthanized 4 days (n = 4) or 10 days (n = 2) post-injection. FDBs were dissected and force production was recorded for control and injured muscles using a force-frequency curve protocol. FDBs tested 10 days post-treatment were flash frozen in OCT, sectioned to 12 μm, and stained with H&E before imaging at ×20. A Four days post-injection absolute force (mN). B Four days post-injection specific force (N/cm2) corrected to PCSA. C Ten days post-injection absolute force (mN). D Ten days post-injection specific force (N/cm2) corrected to PCSA. E PBS-treated FDB muscle section stained with H&E. F CTX-treated FDB muscle section stained with H&E. *, p < 0.05. Data are mean ± SEM