Fig. 6
Comparison of mitochondrial respiration between the FDB and red gastrocnemius muscle. Permeabilized FDB and gastrocnemius muscle bundles were prepared for high-resolution respirometry (n = 4). Substrates and inhibitors were added sequentially to measure CI leak and ADP-stimulated CI, CI + II, CII, and CIV mitochondrial respiration. A Comparison of mitochondrial respiration in intact permeabilized red gastrocnemius and FDB muscle bundles corrected to dry weight. B Inset of graph A showing FDB mitochondrial respiration only. C Comparison of mitochondrial respiration in intact permeabilized red gastrocnemius and FDB muscle bundles corrected to total protein content. D Comparison of mitochondrial respiration in intact permeabilized red gastrocnemius and FDB muscle bundles corrected to CS activity. E Comparison of CS activity between red gastrocnemius and FDB muscle. CI leak, complex I leakstate4; CI, complex I respirationstate3ADP; CI + II, complex I and II respirationstate3ADP; CII, complex II respirationstate3ADP; CIV, super-complex respiration. *, p < 0.05. Data are mean ± SEM