Fig. 8

Electroporation of PGC1α cDNA increased mitochondrial respiration. GFP-conjugated PGC1α and YFP cDNA were electroporated into one FDB and a contralateral control FDB, respectively (n = 7). Mice were euthanized 14 days later and whole FDB muscle was imaged using fluorescent microscopy to confirm protein expression before intact permeabilized FDB muscle bundles were prepared for assessment of mitochondrial respiration between FDBs overexpressing Pgc1α or YFP. A YFP expression in whole FDB 14 days post cDNA electroporation; images were taken with a 488-nm filter cube and in phase contrast then merged to show transfection efficiency. B GFP-conjugated Pgc1α expression in whole FDB 14 days post cDNA electroporation; images were taken with a 488-nm filter cube and in phase contrast then merged to show transfection efficiency. C Comparison of CS activity between YFP and Pgc1α overexpressing FDB muscle bundles. D Overview of mitochondrial respiration across ETC protein complexes between YFP and Pgc1α overexpressing FDB muscle bundles, corrected to muscle dry weight. E Overview of mitochondrial respiration across ETC protein complexes between YFP and Pgc1α overexpressing FDB muscle bundles, corrected to CS activity. CI leak, complex I leak^{state 4}; CI, complex I respiration^state3ADP; CI + II, complex I and II respiration^state3ADP; CII, complex II respiration^state3ADP; CIV, super-complex respiration. * p < 0.05. Data are mean ± SEM