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Fig. 4 | Skeletal Muscle

Fig. 4

From: miR-708-5p and miR-34c-5p are involved in nNOS regulation in dystrophic context

Fig. 4

miR-31, miR-708, miR-34c, and nNOS expression in DMDd45-52 myoblasts. a miRNA expression in control human myoblasts and DMDd45-52 human myoblasts. Graph represents relative quantification of miRNA normalized on SNORD44 expression. miR-708 n = 7, miR-31 n = 7, and miR-34c n = 8. b nNOS immunoblot in control and DMDd45-52 cells. GAPDH serves as the loading control. Bar graph shows quantification results average of 8 independent experiments. c Control myoblasts immunolabeled with anti-nNOS (red) antibody, nuclei with Dapi (blue), and imaged by confocal microscopy. Scale bars, 10 μm. Representative of 3 independent experiments. *p ≤ 0.05

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