Fig. 6
From: miR-708-5p and miR-34c-5p are involved in nNOS regulation in dystrophic context
Inhibition of miR-708 and miR-34c increased nNOS expression in transfected DMDd45-52 human myoblasts. a miRNA expression in DMDd45-52 human myoblasts transfected with control non-specific miRNA (miR-neg, black bar) or indicated selected antimiR (gray bar). Graph represents average of relative quantification of miRNA normalized on SNORD44 expression of 6 (antimiR-708) or 3 (antimiR-34c) independent experiments. b DMDd45-52 myoblasts immunolabeled with anti-nNOS (red) antibody, nuclei with Dapi (blue), and imaged by confocal microscopy. Scale bars, 10 μm. Representative of 5 independent experiments. c nNOS immunoblot in transfected DMDd45-52 human myoblasts. GAPDH serves as the loading control. Bar graph shows quantification results average of 5 independent experiments. *p ≤ 0.05