Table 2 Primers used to fragment NOS1-3′UTR
From: miR-708-5p and miR-34c-5p are involved in nNOS regulation in dystrophic context
Position in 3′UTR) | Forward primer | Reverse primer |
|---|---|---|
Part 1 (1-1896) | F1-5′TTGTCTAGACTGGACCCTCTTGCCCAGC-3′ | R1-5′AAGGATATCCAGGGGAAATTGGGATTAAAGG-3′ |
Part 2 (1773-3685) | F2-5′-AACTCTAGACTATGACTCACCTTGCTCTGC-3′ | R2-5′-ATCGATATCCTTACATGCTCCCTGTCCGTG-3′ |
Part 3 (3607-5523) | F3-5′-AATTCTAGACTGGTAGCTTCTGGAAGGTAAG-3′ | R3-5′AATGATATCGCCACAAGGCAGGGACTGGC-3′ |
Part 4 (5358-7149) | F4-5′-TAGTCTAGAGAAACACAGGTCTGAGGGTCTG-3′ | R4-5′-CCGATATCATTGTAACCATAATGCAAACAAGC-3′ |