Fig. 1

Muscle stem cell numbers are reduced in ΔKlotho mice. a–c Representative immunofluorescence images of TA muscle cross-sections from ΔKlotho and control mice stained for DAPI (DNA, blue) and Laminin (green), and Pax7 (red) at a p56, b p14, and c p21. Scale bar = 50 μm. d Quantification of Pax7-positive SCs on whole TA cross-sections from ΔKlotho and control mice at p14, p21, and p56. n = 3 mice per genotype and time point. All data are presented as means ± SEM. *p < 0.05