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Fig. 3 | Skeletal Muscle

Fig. 3

From: Klotho expression is a prerequisite for proper muscle stem cell function and regeneration of skeletal muscle

Fig. 3

Late regeneration of skeletal muscle is impaired in ΔKlotho mice. a The right TA muscle from ΔKlotho and control mice was injured by injection of cardiotoxin (CTX) at p39 and isolated at p60. b TA muscles (arrows) show signs of impaired regeneration by H&E staining and extensive fibrosis in ΔKlotho mice at day 21 after injury (c), but not in control littermates as demonstrated by Sirius Red staining. Alizarin Red marks calcifications, visible in regenerating muscles from ΔKlotho mice as well as lipid accumulations shown by Oil Red O staining. Scale bar = 100 μm. d Minimal fiber feret measured on whole cross-sections of TA muscles from ΔKlotho and control at d21 after injury. (ΔKlotho n = 4 mice, control n = 4 mice). e Immunostainings of cross-sections of TA muscles at d21 after injury with antibodies directed against laminin (green) and developmental (dev) MHC (red), nuclei are stained with DAPI (DNA, blue). Scale bar = 50 μm. Proportion of (f) devMHC-positive fibers (ΔKlotho n = 2 mice, control n = 4 mice) and (g) Pax7-positive satellite cells per myofiber on whole cross-sections at d21 after CTX-induced injury. (ΔKlotho n = 4 mice, control n = 4 mice). h Representative images of stainings for CD68 (in red) and laminin (in green) showing increased infiltration with macrophages in regenerating muscles from ΔKlotho mice. Scale bar = 50 um. All data are presented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001

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