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Fig. 6 | Skeletal Muscle

Fig. 6

From: Klotho expression is a prerequisite for proper muscle stem cell function and regeneration of skeletal muscle

Fig. 6

sKlotho antagonizes aberrant Wnt3a function in aged muscle stem cells. a αKlotho mRNA expression in myofibers with their adjacent muscle stem cells directly after isolation or after 72 h of culture from young and old C57BL/6J mice (young mice: n = 4, old mice: n = 5). b Addition of sKlotho to primary myoblasts reduces induction of canonical Wnt signaling evoked by addition of recombinant Wnt3a. c Myofibers with their adjacent muscle stem cells from young (4 months) and old (22–24 months) mice were cultured for 72 h with normal medium, recombinant soluble klotho (KL) protein, recombinant Wnt3a, or recombinant Dkk1. (n = 6 mice (young), n = 5 (old)). The number of clusters per myofiber was normalized to young control. d Myofibers with their adjacent muscle stem cells from young (4 months) mice were cultured for 72 h with normal medium, recombinant soluble klotho (KL) protein, recombinant Wnt3a, or a combination of both (n = 6 mice). The number of clusters per myofiber was normalized to young control. e Addition of sKlotho recombinant protein (KL) increases the proportion of Pax7+/MyoD− cells per fiber located in a cluster. The number of Pax7+/MyoD− cells (located in a cluster) per myofiber was normalized to young control (n = 6 mice (young), n = 5 (old)). f Myofibers with their adjacent muscle stem cells from young (4 months) mice were cultured for 72 h with normal medium, recombinant soluble klotho (KL) protein, recombinant Wnt3a, or a combination of both (n = 6 mice). The number of Pax7+/MyoD− cells (located in a cluster) per myofiber was normalized to young control. All data are presented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001

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