Fig. 3

Three-dimensional reconstructions of perfusion-based CUBIC cleared rat organs. a Cerebellum, 3D view from different angles and (a1–a4) single slices. Single sections represent foil of cerebellum (a1), vermis (a2), medullary velum (a3), and cerebellar peduncle (a4). b Heart reconstruction presenting the auricles, aorta, and coronary vessels (arrowhead). Single sections represent the auricle (b1), great arteries (b2), mitral and pulmonary valve (b3), and left ventricle (b4). c Abdominal aorta bifurcation into the left and right common iliac arteries. Single sections represent the arteries: renal (c1), inferior mesenteric (c2), common iliac (c3), and external iliac (c4). Scale bar, 1 mm